내부 동원체 단백질은 INCENP [5] [6] [7] 유전자 에 의해 인간에서 암호화되는 단백질 이다.
포유류의 세포에서, 동원체 상호작용 단백질의 두 가지 광범위한 그룹이 설명되었다: 구성적 으로 동원체 단백질과 '승객'[8] 구성 단백질은 CENPA (중심단백질 A), CENPB , CENPC1 , CENPD 를 포함한다.
'승객단백질'이라는 용어는 [9] 여기에는 CENPE, MCAK , KID , 세포질 다이네인(예: DYNC1H1 ), CliPs(예 : CLIP1) 및 CENPF/미토신(CENPF)이 포함 된다. 승객 단백질 그룹의 초기 구성원인 내부 동원체 단백질(INCENPs)[5] 유사분열 초기 염색체를 따라 광범위한 국재성을 나타내지만 세포 주기가 중간 변형 단계로 진행됨에 따라 동원체에 점차 집중된다. 말단기 동안, 단백질은 세포간 교량의 중간체 안에 위치하고, 그곳에서 사이토키네시스 [7] [10]
INCENP는 염색체 승객 복합체의 조절 단백질이다. 이는 촉매 단백질 Aurora B의 조절에 관여합니다. 그것 은 두 개의 다른 단백질 인 Survivin과 Borealin 과 함께 이 기능을 수행합니다.이 단백질들은 촘촘한 세 개의 나선형 다발을 형성한다.INCENP의 N 터미널 도메인은 이 3개의 헬리컬번들의 [11]
상호 작용 INCENP는 H2AFZ ,[12] Survivin [13] CDCA8 과 [14] 것 으로 나타났습니다. ARK 결합 영역은 오로라 관련 키나제에 결합하는 데 필요하고 충분한 것으로 밝혀졌다. 이 상호작용은 [15] 효모 의 세포분열 과 염색체 분리의 조정과 관련이 있다.
레퍼런스 ^ a b c GRCh38: 앙상블 릴리즈 89: ENSG00000149503 - 앙상블 , 2017년 5월^ a b c GRCm38: 앙상블 릴리즈 89: ENSMUSG000024660 - 앙상블 , 2017년 5월^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ a b Earnshaw WC, Cooke CA (Sep 1991). "Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation". J Cell Sci . 98 (4): 443–61. doi :10.1242/jcs.98.4.443 . PMID 1860899 . ^ Adams RR, Eckley DM, Vagnarelli P, Wheatley SP, Gerloff DL, Mackay AM, Svingen PA, Kaufmann SH, Earnshaw WC (Jul 2001). "Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells". Chromosoma . 110 (2): 65–74. doi :10.1007/s004120100130 . PMID 11453556 . S2CID 13778759 . ^ a b "Entrez Gene: INCENP inner centromere protein antigens 135/155kDa" .^ Choo, K. H. Andy (1997). The centromere ISBN 978-0-19-857780-5 ^ Earnshaw WC, Mackay AM (September 1994). "Role of nonhistone proteins in the chromosomal events of mitosis" . FASEB J . 8 (12): 947–56. doi :10.1096/fasebj.8.12.8088460 . PMID 8088460 . S2CID 14062246 . ^ Cutts SM, Fowler KJ, Kile BT, Hii LL, O'Dowd RA, Hudson DF, Saffery R, Kalitsis P, Earle E, Choo KH (July 1999). "Defective chromosome segregation, microtubule bundling and nuclear bridging in inner centromere protein gene (Incenp)-disrupted mice" . Hum. Mol. Genet . 8 (7): 1145–55. doi :10.1093/hmg/8.7.1145 PMID 10369859 . ^ Jeyaprakash, A. A.; Klein, U. R.; Lindner, D.; Ebert, J.; Nigg, E. A.; Conti, E. (2007). "Structure of a Survivin–Borealin–INCENP Core Complex Reveals How Chromosomal Passengers Travel Together" . Cell . 131 (2): 271–285. doi :10.1016/j.cell.2007.07.045 PMID 17956729 . ^ Rangasamy D, Berven L, Ridgway P, Tremethick DJ (April 2003). "Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development" . EMBO J . 22 (7): 1599–607. doi :10.1093/emboj/cdg160 . PMC 152904 PMID 12660166 . ^ Wheatley SP, Carvalho A, Vagnarelli P, Earnshaw WC (June 2001). "INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis" . Curr. Biol . 11 (11): 886–90. doi :10.1016/S0960-9822(01)00238-X PMID 11516652 . ^ Gassmann R, Carvalho A, Henzing AJ, Ruchaud S, Hudson DF, Honda R, Nigg EA, Gerloff DL, Earnshaw WC (July 2004). "Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle" . J. Cell Biol . 166 (2): 179–91. doi :10.1083/jcb.200404001 . PMC 2172304 PMID 15249581 . ^ Leverson JD, Huang HK, Forsburg SL, Hunter T (April 2002). "The Schizosaccharomyces pombe aurora-related kinase Ark1 interacts with the inner centromere protein Pic1 and mediates chromosome segregation and cytokinesis" . Mol. Biol. Cell . 13 (4): 1132–43. doi :10.1091/mbc.01-07-0330 . PMC 102257 PMID 11950927 .
추가 정보 Ainsztein AM, Kandels-Lewis SE, Mackay AM, Earnshaw WC (1999). "INCENP centromere and spindle targeting: identification of essential conserved motifs and involvement of heterochromatin protein HP1" . J. Cell Biol . 143 (7): 1763–74. doi :10.1083/jcb.143.7.1763 . PMC 2175214 PMID 9864353 . Martineau-Thuillier S, Andreassen PR, Margolis RL (1999). "Colocalization of TD-60 and INCENP throughout G2 and mitosis: evidence for their possible interaction in signalling cytokinesis". Chromosoma . 107 (6–7): 461–70. doi :10.1007/s004120050330 . PMID 9914378 . S2CID 24935187 . Dias Neto E, Correa RG, Verjovski-Almeida S, et al. (2000). "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags" . Proc. Natl. Acad. Sci. U.S.A . 97 (7): 3491–6. Bibcode :2000PNAS...97.3491D . doi :10.1073/pnas.97.7.3491 PMC 16267 PMID 10737800 . Wheatley SP, Kandels-Lewis SE, Adams RR, et al. (2001). "INCENP binds directly to tubulin and requires dynamic microtubules to target to the cleavage furrow". Exp. Cell Res . 262 (2): 122–7. doi :10.1006/excr.2000.5088 . PMID 11139336 . Wheatley SP, Carvalho A, Vagnarelli P, Earnshaw WC (2001). "INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis" . Curr. Biol . 11 (11): 886–90. doi :10.1016/S0960-9822(01)00238-X PMID 11516652 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Parra MT, Viera A, Gómez R, et al. (2003). "Dynamic relocalization of the chromosomal passenger complex proteins inner centromere protein (INCENP) and aurora-B kinase during male mouse meiosis" . J. Cell Sci . 116 (Pt 6): 961–74. doi :10.1242/jcs.00330 PMID 12584241 . Rangasamy D, Berven L, Ridgway P, Tremethick DJ (2003). "Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development" . EMBO J . 22 (7): 1599–607. doi :10.1093/emboj/cdg160 . PMC 152904 PMID 12660166 . Honda R, Körner R, Nigg EA (2004). "Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis" . Mol. Biol. Cell . 14 (8): 3325–41. doi :10.1091/mbc.E02-11-0769 . PMC 181570 PMID 12925766 . Wheatley SP, Henzing AJ, Dodson H, et al. (2004). "Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo" . J. Biol. Chem . 279 (7): 5655–60. doi :10.1074/jbc.M311299200 PMID 14610074 . Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs" . Nat. Genet . 36 (1): 40–5. doi :10.1038/ng1285 PMID 14702039 . Gassmann R, Carvalho A, Henzing AJ, et al. (2004). "Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle" . J. Cell Biol . 166 (2): 179–91. doi :10.1083/jcb.200404001 . PMC 2172304 PMID 15249581 . Li X, Sakashita G, Matsuzaki H, et al. (2004). "Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C" . J. Biol. Chem . 279 (45): 47201–11. doi :10.1074/jbc.M403029200 PMID 15316025 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Zhu C, Bossy-Wetzel E, Jiang W (2005). "Recruitment of MKLP1 to the spindle midzone/midbody by INCENP is essential for midbody formation and completion of cytokinesis in human cells" . Biochem. J . 389 (Pt 2): 373–81. doi :10.1042/BJ20050097 . PMC 1175114 PMID 15796717 . Chen HL, Tang CJ, Chen CY, Tang TK (2005). "Overexpression of an Aurora-C kinase-deficient mutant disrupts the Aurora-B/INCENP complex and induces polyploidy". J. Biomed. Sci . 12 (2): 297–310. doi :10.1007/s11373-005-0980-0 . PMID 15917996 . Vader G, Kauw JJ, Medema RH, Lens SM (2006). "Survivin mediates targeting of the chromosomal passenger complex to the centromere and midbody" . EMBO Rep . 7 (1): 85–92. doi :10.1038/sj.embor.7400562 . PMC 1369225 PMID 16239925 . Goto H, Kiyono T, Tomono Y, et al. (2006). "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition". Nat. Cell Biol . 8 (2): 180–7. doi :10.1038/ncb1350 . PMID 16378098 . S2CID 11583385 .
